Incorporating　security　labels　with　physical　unclonable　functions　(PUFs)　into　products　is　considered　as　the　most　effective　anti-counterfeiting　measure.　Here　we　reported　a　PUF-based　security　label　composed　of　random　colorful　patterns.　The　security　label　was　fabricated　through　self-assembly　of　coordination　polymers-a　cupric　bromide　complex　of　pyrazine　1,4-dioxide-on　lithographically　defined　plasmonic　surfaces.　The　coordination　polymer　exhibits　excitation　wavelength　dependent　fluorescence　fingerprints,　which　enables　encoding　spectral　information　in　the　polymer.　The　fluorescence　peaks　of　each　spectrum　can　be　easily　converted　to　a　unique　anti-counterfeiting　barcode.　The　developed　security　label　also　carries　2D　graphical　information,　which　can　be　visualized　under　bright-field,　dark-field　and　fluorescence　multifunctional　microscopy.　It　is　multicolored　with　high　contrast　under　the　bright/dark-field　modes　and　single　color　under　fluorescence　mode.　The　multicolors　originate　from　polymer-thickness　dependent　optical　interference.　The　encryption　with　colors　on　a　50　μm　square　produces　a　minimum　of　3739　PUF　codes　for　the　realized　authentication　system.　Moreover,　we　developed　a　convenient　and　reliable　authentication　strategy　based　on　the　computer　vision　technique　to　verify　the　colorful　PUF　patterns　with　different　definition,　rotation　angles,　brightness,　magnification　and　a　mixture　of　these　factors.　A　high　correct　validation　rate　of　100%　is　achieved　using　a　match　threshold　of　0.3.　©　The　Royal　Society　of　Chemistry　2019.