Herein,　we　propose　an　aptamer-equipping　strategy　to　generate　specific,　universal　and　permeable　(SUPER)　NK　cells　for　enhanced　immunotherapy　in　solid　tumors.　NK　cells　were　chemically　equipped　with　TLS11a　aptamer　targeting　HepG2　cells　and　PDL1-specific　aptamer　without　genetic　alteration.　The　dual　aptamer-equipped　NK　cells　exhibited　high　specificity　to　tumor　cells,　resulting　in　higher　cytokine　secretion　and　apoptosis/necrosis　compared　to　parental　or　single　aptamer-equipped　NK　cells.　Interestingly,　dual　aptamer-equipped　NK　cells　induced　remarkable　upregulation　of　PDL1　expression　in　HepG2　cells,　enhancing　checkpoint　blockade.　Furthermore,　in　vivo　intravital　imaging　demonstrated　high　infiltration　of　aptamer-equipped　NK　cells　into　deep　tumor　region,　leading　to　enhanced　therapeutic　efficacy　in　solid　tumors.　This　work　offers　a　straightforward　chemical　strategy　to　equip　NK　cells　with　aptamers,　holding　considerable　potential　for　enhanced　adoptive　immunotherapy　in　solid　tumors.　©　2020　Wiley-VCH　Verlag　GmbH　&　Co.　KGaA,　Weinheim