The　study　aims　to　evaluate　research　trends　of　ecotourism,　which　has　attracted　wide　attention　by　both　researchers　and　policymakers　as　an　important　way　to　realize　sustainable　tourism.　Bibliometric　analysis　was　carried　through　the　2,531　records　related　to　ecotourism　searching　from　Science　Citation　Index　(SCI),　Social　Science　Citation　Index　(SSCI),　Arts　&　Humanities　Citation　Index　(A&HCI),　and　Index　to　Scientific　&　Technical　Proceedings　(ISTP)　databases　from　1990　to　2016.　A　total　of　4,138　keywords　were　divided　into　three　categories:　topical　keywords,　case-related　keywords,　and　research　methods-related　keywords.　They,　respectively,　were　analyzed　on　overall　development　as　well　as　the　dynamic　changes　by　three　divided　stages　of　ecotourism　research.　The　results　showed　that　Tourism　Management　was　the　most　prolific　journal.　U.S.-based　authors　published　more　often　than　those　from　any　other　country,　whereas　among　research　institutions,　the　Chinese　Academy　of　Sciences　was　the　most　prolific　contributor.　It　also　revealed　that　conservation　was　the　key　focus,　protected　area　was　the　main　study　object,　and　sustainable　tourism　was　the　main　aim.　The　top　three　countries　for　cases　study　were　China,　the　United　States,　and　South　Africa,　and　the　main　methodologies　were　contingent　valuation　method　and　geographic　information　system.　According　to　the　dynamic　analysis,　research　perspectives　changed　from　ecotourism　resources　to　management　and　subsequently　expanded　to　multistakeholders　involvement,　with　a　rapid　expansion　of　the　case　and　implementation　of　quantitative　research　methods.　With　multidisciplinary　involvement　and　multistakeholders　participation,　the　study　of　tourist　awareness　and　behavior　on　ecotourism　would　become　the　main　aspects　for　deepening　and　concreting　ecotourism　research.　Our　findings　provide　a　quantitative　understanding　of　global　ecotourism　research　features.