Amphibian　defensive　skin　secretions　are　complex,　species-specific　cocktails　of　biologically　active　molecules,　including　many　uncharacterized　peptides.　The　study　of　such　secretions　for　novel　peptide　discovery　is　time-limited,　as　amphibians　are　in　rapid　global　decline.　While　secretion　proteome　analysis　is　non-lethal,　transcriptome　analysis　has　until　now　required　killing　of　specimens　prior　to　skin　dissection　for　cDNA　library　construction.　Here　we　present　the　discovery　that　polyadenylated　mRNAs　encoding　dermal　granular　gland　peptides　are　present　in　defensive　skin　secretions,　stabilized　by　endogenous　nucleic　acid-binding　amphipathic　peptides.　Thus　parallel　secretory　proteome　and　transcriptome　analyses　can　be　performed　without　killing　the　specimen　in　this　model　amphibian　system　-　a　finding　that　has　important　implications　in　conservation　of　biodiversity　within　this　threatened　vertebrate　taxon　and　whose　mechanistics　may　have　broader　implications　in　biomolecular　science.