Objective:　To　review　the　feasibility　of　coupling　the　techniques　of　random　amplified　polymorphic　DNA　(RAPD)　with　carbon　nanotube-based　modified　electrode　for　guanine/deoxy-guanine　triphosphate　(dGTP)　electrochemical　sensing　for　mapping　of　the　pancreatic　cancer　genetic　fingerprint　and　screening　of　genetic　alterations.　Methods:　We　developed　a　new　method　to　study　the　electrochemical　behavior　of　dGTP　utilizing　carbon　multiwalled　nanotube　(MWNT)-modified　glassy　carbon　electrodes　(GCEs).　RAPD　was　applied　for　amplification　of　DNA　samples　from　healthy　controls　and　patients　with　pancreatic　cancer　under　the　same　conditions　to　determine　the　different　surplus　quantity　of　dGTP　in　the　polymerase　chain　reaction　(PCR),　thereby　determining　the　difference/quantity　of　PCR　products　or　template　strands.　Using　this　method　we　generated　a　genetic　fingerprint　map　of　pancreatic　cancer　through　the　combination　of　electrochemical　sensors　and　gel　electrophoresis　to　screen　for　genetic　alterations.　Cloning　and　sequencing　were　then　performed　to　verify　these　gene　alterations.　Results:　dGTP　showed　favorable　electrochemical　behavior　on　the　MWNTs/GCE.　The　results　indicated　that　the　electrical　signal　and　dGTP　had　a　satisfactory　linear　relationship　with　the　dGTP　concentration　within　the　conventional　PCR　concentration　range.　The　MWNTs/GCE　could　distinguish　between　different　products　of　RAPD.　This　experiment　successfully　identified　a　new　pancreatic　cancer-associated　mutant　gene　fragment,　consisting　of　a　cyclin-dependent　kinase　4　gene　3＇　terminal　mutation.　Conclusion:　The　coupling　of　RAPD　and　nanoelectrochemical　sensors　was　successfully　applied　to　the　screening　of　genetic　alterations　in　pancreatic　cancer　and　for　mapping　of　DNA　fingerprints.