A　novel　fungi　pelletization-assisted　bioflocculation　technology　was　developed　for　efficient　algae　harvesting　and　wastewater　treatment.　Microalga　UMN235　and　two　locally　isolated　fungal　species　UMN　F01　and　UMN　F02　were　used　to　study　the　effect　of　various　cultural　conditions　on　pelletization　process　for　fungi-algae　complex.　The　results　showed　that　pH　was　the　key　factor　affecting　formation　of　fungi-algae　pellet,　and　pH　could　be　controlled　by　adjusting　glucose　concentration　and　fungal　spore　number　added.　The　best　pelletization　happened　when　adding　20　g/L　glucose　and　approximately　1.2E8/L　spores　in　BG-11　medium,　under　which　almost　100　%　of　algal　cells　were　captured　onto　the　pellets　with　shorter　retention　time.　The　fungi-algae　pellets　can　be　easily　harvested　by　simple　filtration　due　to　its　large　size　(2-5　mm).　The　filtered　fungi-algae　pellets　were　reused　as　immobilized　cells　for　treatment　wastewaters　and　the　nutrient　removal　rates　of　100,　58.85,　89.83,　and　62.53　%　(for　centrate)　and　23.23,　44.68,　84.70,　and　70.34　%　(for　diluted　swine　manure　wastewater)　for　ammonium,　total　nitrogen,　total　phosphorus,　and　chemical　oxygen　demand,　respectively,　under　both　1-　and　2-day　cultivations.　The　novel　technology　developed　is　highly　promising　compared　with　current　algae　harvesting　and　biological　wastewater　treatment　technologies　in　the　literature.