A　novel　human　papillomavirus　(HPV)　typing　assay　for　cervical　cancer　screening　and　prognosis　was　developed　by　the　combination　of　restriction　fragment　length　polymorphism　(RFLP)　and　microchip　electrophoresis　(MCE)　to　achieve　higher　levels　of　sensitivity　and　throughput.　The　detection　limit　of　2　x　10(2)　copies,　high　sensitivity　and　typing　accuracy　on　the　account　of　PCR-RFLP-MCE　method　guarantee　the　successful　diagnosis　results　of　4-fold　higher　infection　rate　over　cytologic　tests.　From　clinical　samples,　eleven　kinds　of　HPV　types　were　identified　with　a　good　compatibility　degree　of　over　90%.　The　described　method　showed　good　reliability　in　clinical　samples　and　provided　a　promising　alternative　for　pathological　studies　at　the　molecular　level.