Soot　concentration　(KL　factor)　and　soot　temperature　were　measured　in　a　constant　volume　combustion　chamber　for　a　new　biomass-based　biofuel　or　BTL　(biomass　to　liquid)　fuel　and　regular　No.2　diesel.　A　highspeed　camera　was　employed　coupled　with　two　bandpass　filters　to　implement　a　two-color　thermometry　method　and　measure　the　soot　concentration　and　temperature　simultaneously.　Ambient　conditions　were　set　as　follows:　three　temperatures　of　800　K,　1000　K,　and　1200　K　and　four　02　concentrations　of　10%,　15%,　18%　and　21%.　The　soot　ML　factor　and　temperature　spatial　distributions　are　presented　for　1000　K　ambient　temperature.　More　soot　is　seen　in　the　near-wall　regions　under　the　low　ambient　oxygen　conditions　while　high　level　soot　is　observed　in　the　upstream　and　midstream　for　the　conventional　combustion　mode.　An　analysis　was　then　conducted　for　the　quasi-steady　state.　The　results　show　that　BTL　combustion　generates　a　lower　integrated　ML　factor　and　soot　temperature　compared　to　diesel　fuel　under　all　the　experimental　conditions.　Additionally,　low　ambient　temperature　with　a　moderate　02　concentration　benefits　BTL　more　than　diesel　due　to　a　larger　reduction　in　the　integrated　la.　factor　without　increasing　soot　temperature　significantly.　Finally,　the　characteristics　of　the　two-color　results　were　further　discussed　and　analyzed.　(C)　2015　Elsevier　Ltd.　All　rights　reserved.