This　research　aims　to　prepare　the　biphasic-induced　magnetic　composite　microcapsules　(BIMCM)　as　a　promising　environmental　stimuli-responsive　delivery　vehicle　to　dispose　the　problem　of　drug　burst　effect.　The　paper　presented　a　novel　automated　in　situ　click　technology　of　magnetic　chitosan/nano　hydroxyapatite　(CS/nHA)　microcapsules.　Fe3O4　magnetic　nanoparticles　(MNP)　and　nHA　were　simultaneously　in　situ　crystallized　by　one-step　process.　Icariin　(ICA),　a　plant-derived　flavonol　glycoside,　was　combined　to　study　drug　release　properties　of　BIMCM.　BIMCM　were　characterized　by　Fourier　transform　infrared　spectroscopy　(FTIR),　X-ray　diffraction　(XRD),　X-ray　photoelectron　spectroscopy　(XPS),　scanning　electron　microscopy.(SEM),　and　Thermal　gravimetric　analysis/Differential　Scanning　Calorimetry(TGA/DSC)　in　order　to　reveal　their　component　and　surface　morphology　as　well　as　the　role　of　the　in　situ　generated　Fe3O4　MNP　and　nHA.　The　magnetic　test　showed　the　BIMCM　were　super-paramagnetic.　Both　in　situ　generated　Fe3O4　MNP　and　nHA　serve　as　stable　inorganic　crosslinkers　in　BIMCM　to　form　many　intermolecular　crosslinkages　for　the　movability　of　the　CS　chains.　This　makes　ICA　loaded　microcapsules　take　on　a　sustained　release　behavior　and　results　in　the　self-adjusting　of　surface　morphology,　decreasing　of　swelling　and　degradation　rates.　In　addition,　in　vitro　tests　were　systematically　carried　out　to　examine　the　biocompatibility　of　the　micro　capsules　by　KIT　test,　Wright-Giemsa　dying　assay　and　AO/EB　fluorescent　staining　method.　These　results　demonstrated　that　successful　introduction　of　the　in　situ　click　Fe3O4　MNP　provided　an　alternative　strategy　because　of　magnetic　sensitivity　and　sustained　release.　As　such,　the　novel　ICA　loaded　biphasic-induced　magnetic　CS/nHA/MNP　microcapsules　are　expected　to　find　potential　applications　in　drug　delivery　system　for　bone　repair.　(C)　2015　Elsevier　B.V.　All　rights　reserved.