As　one　of　the　stimulators　on　bone　formation,　osteogenic　growth　peptide　(OGP)　improves　both　proliferation　and　differentiation　of　the　bone　cells　in　vitro　and　in　vivo.　The　aim　of　this　work　was　the　preparation　of　three　dimensional　porous　poly(lactic　acid)　(PLA)　scaffold　with　high　porosity　from　PLA-dioxane-water　ternary　system　with　the　use　of　vacuum-assisted　solvent　casting,　phase　separation,　solvent　extraction　and　particle　leaching　methods.　Then,　by　surface　coating　of　PIA　scaffold　with　chitosan　(CS)/OGP　solution,　biofunctionalization　of　PLA　scaffold　had　been　completed　for　application　in　bone　regeneration.　The　effects　of　frozen　temperature　(-20,　-50,　-80　degrees　C)　and　PLA　solution　concentration　(10,　12,　14　wt%)　on　the　microstructure,　water　absorption,　porosity,　hydrophilicity,　mechanical　properties,　and　biocompatibility　of　PIA　and　CS/OGP/PLA　scaffold　were　investigated.　Results　showed　that　both　PIA　and　CS/OGP/PLA　scaffolds　have　an　interconnected　network　structure　and　a　porosity　of　up　to　96.1%　and　91.5%,　respectively.　The　CS/OGP/PLA　scaffold　exhibited　better　hydrophilicity　and　mechanical　properties　than　that　of　uncoated　PLA　scaffold.　Moreover,　the　results　of　cell　culture　test　showed　that　CS/OGP　coating　could　stimulate　the　proliferation　and　growth　of　osteoblast　cells　on　CS/OGP/PLA　scaffold.　These　finding　suggested　that　the　surface　biofunctionalization　by　CS/OGP　coating　layer　could　be　an　effective　method　on　enhancing　cell　adhesion　to　synthetic　polymer-based　scaffolds　in　tissue　engineering　application　and　the　developed　porous　CS/OGP/PLA　scaffold　should　be　considered　as　alternative　biomaterials　for　bone　regeneration.　(C)　2017　Elsevier　B.V.　All　rights　reserved.