Two　new　diketopiperazine　alkaloids,　named　protuboxepin　C　(1)　and　protuboxepin　D　(2),　which　contain　D-Phe　residue　and　oxepin　ring,　were　isolated　from　EtOAc　extract　of　sponge-derived　fungus　Aspergillus　sp　SCSIO　XWS02F40.　Their　structures　were　elucidated　by　1D,　2D　NMR　and　HRESIMS　dates,　and　their　absolute　configurations　were　confirmed　by　single　crystal　X-ray　diffraction　experiments　and　CD　analyses.　The　in　vitro　cytotoxicity　of　these　two　new　compounds　was　further　evaluated　using　A549　and　Hela　cell　lines.　[GRAPHICS]　.