Mesoporous　silica　microspheres　were　prepared　by　the　polymerization-induced　colloid　aggregation　(PICA)　and　pseudomorphic　synthesis　methods.　The　prepared　microspheres　have　high　specific　surface　area　and　MCM-41　type　structure.　In　the　PICA　process,　acidic　silica　sol　was　utilized　as　silica　source　and　the　effect　of　molar　ration　(formaldehyde/urea)　was　investigated.　Moreover,　the　influences　of　reaction　time　and　temperature　were　also　studied.　The　specific　surface　area　of　porous　and　mesoporous　silica　microspheres　were　186.4　m(2)/g　and　900.4　m(2)/g,　respectively.　The　materials　were　characterized　by　SAXS,　FTIR,　SEM,　TEM　and　nitrogen　sorption　measurements.　The　prepared　silica　microspheres　were　functionalized　by　(3-Aminopropyl)triethoxysilane　and　then　used　to　remove　the　lead　from　aqueous　solution.　The　result　indicates　that　the　grafted　silica　microspheres　have　rapid　adsorption　capacity　and　good　reproducibility.　The　adsorption　data　was　fitted　well　with　the　Langmuir　isotherm　model,　and　the　maximum　adsorption　capacities　for　MCM-41　silica　microspheres　were　102.7　mg/g.