A　novel　polyhedral　oligomeric　silsesquioxane　(POSS)-containing　aptamer-modified　hybrid　affinity　monolith　with　excellent　hydrophilicity　and　unique　architecture　without　Si-OH　groups　is　presented　herein,　and　the　nonspecific　adsorption　caused　by　the　hydrophobic　nature　of　the　monolithic　column　or　polar　interaction　with　silanol　groups　is　minimized.　Via　a　simple　one-pot　procedure,　hydrophilic　monomers　were　facilely　polymerized　with　the　POSS-methacryl　substituted　(POSS-MA)　and　aptamer;　a　highly　hydrophilic　nature　was　obtained　and　the　lowest　contact　angle　of　11　degrees　was　achieved.　By　using　ochratoxin　A　(OTA)　as　the　model　analyte,　highly　selective　recognition　of　OTA　in　the　mixture　was　achieved　and　the　control　of　nonspecific　interactions　and　the　cross-reactivity　of　OTB　and　AFB(1)　were　significantly　improved.　The　recovery　yield　of　OTB　caused　by　nonspecific　adsorption　in　the　resultant　monolith　was　only　about　0.1%　and　remained　steady　even　with　the　coexistence　of　a　high　OTB　content　(OTA:OTB　=　1:50),　which　reached　the　best　level　to　date　and　was　obviously　less　than　the　6.1%　occurring　in　the　hydrophobic　POSS-containing　control　monolith,　8.3%　in　the　POSS-PEI@AuNPs@aptamer　affinity　monolith　and　18.7%　in　the　silica-hybrid　affinity　monolith.　When　applied　to　wine　and　wheat　samples,　the　nonspecific　adsorption　was　significantly　reduced　and　efficient　discrimination　of　OTA　was　obtained　with　better　results　than　that　of　the　hydrophobic　POSS-containing　affinity　column.　This　provides　an　attractive　tool　for　minimizing　the　nonspecific　adsorption　for　highly　selective　on-column　recognition.