Photodynamic　antifungal　therapy　is　a　promising　treatment　for　increasing　drug-resistant　fungi.　However,　low　physiological　solubility　and　low　fungi-affinity　of　most　potential　photosensitizers　limits　their　therapeutic　efficacy.　To　improve　the　water-solubility　and　photodynamic　antifungal　activity　of　zinc(II)　phthalocyanine,　two　molecular-weight　carboxymethyl　chitosans　(CMC1,50　kDa;　CMC2,170　kDa)　were　herein　respectively　conjugated　with　1-[4-(2-aminoethyl)phenoxy]　zinc(II)　phthalocyanine　(ZnPcN)　and　further　quaternized,　and　eight　novel　conjugates　were　obtained　and　characterized.　Their　photophysical　and　photochemical　properties,　cellular　uptakes　and　in　vitro　photodynamic　antifungal　activities　against　Candida　albicans　have　also　been　investigated.　All　the　conjugates　are　less　aggregated　in　water　than　ZnPcN.　The　low-molecular-weight　CMC1-conjugated　ZnPcN　is　more　readily　ingested　and　highly　photoactive.　Mainly　due　to　its　highest　uptake　by　Candida　cells,　a　conjugate　of　CMC1　and　ZnPcN　shows　the　highest　photocytotoxicity　with　an　IC90　value　down　to　0.72　mu　M.　Further　quaternization　decreases　the　photocytotoxicity.　Additionally,　the　conjugates　show　special　affinity　to　the　mitochondria　of　C.　albicans.