Via　the　facile　photopolymerization　and　thiol-ene　click　chemistry,　a　hydrophilic　polyhedral　oligomeric　silsesquioxane　(POSS)-containing　affinity　monolithic　column　with　a　high　load　of　aptamers　and　fast　preparation　was　presented,　and　successfully　applied　to　the　efficient　specific　recognition　and　sensitive　detection　of　zearalenone　(ZEN)　by　coupling　with　HPLC.　Optimization　of　polymerization　reaction　and　polymer　recipe　were　studied,　and　characteristics　such　as　structural　morphology,　affinity　performance　and　specificity　to　ZEN　were　evaluated.　A　highly　hydrophilic　POSS-based　aptamer　affinity　monolith　was　rapidly　prepared　in　1.1　h,　and　a　high　capacity　of　aptamer　reached　2772.61　pmol/mu　L　that　was　much　higher　than　that　of　the　current　POSS-based　aptamer　affinity　monoliths.　By　this　way,　the　hydrophilic　nature　and　aptamer　coverage　indensity　in　POSS　monoliths　were　well　improved.　The　mixed-mode　mechanism　including　aptamer　affinity　recognition　and　hydrophilic　interaction　were　employed　for　the　specific　analysis　of　ZEN.　Applied　to　the　monitoring　of　ZEN,　a　good　selective　recognition　and　sensitive　detection　were　obtained　with　the　detection　limit　(LOD)　of　0.02　ng/mL.　The　stability　and　reproducibility　were　acceptable　and　the　intra-day,　inter-day　and　column-to-column　relative　standard　deviations　(RSDs)　of　the　ZEN　recovery　were　gained　in　the　range　from　2.28%　to　5.32%,　respectively.　Satisfactory　recoveries　of　ZEN　in　rice　samples　were　realized　ad　98.6　+/-　2.8%-100.8　+/-　2.0%.　It　might　provide　a　preferable　access　to　online　specific　analysis　of　ZEN　via　the　hydrophilic　POSS-based　affinity　monolith　with　a　large　capacity　of　aptamers　and　a　high　preparation　efficiency.