A　three-dimensional　micromixer　is　designed　and　fabricated　by　using　glass-poly(dimethylsiloxane)　(PDMS)　hybridized　materials.　The　improvement　of　the　fabrication　process　makes　the　micromixer　endure　much　higher　flow　rate.　Based　on　the　self-rotation　effect　of　the　fluid,　the　fast　mixing　can　be　achieved.　The　mixing　process　is　evaluated　by　connecting　the　micromixer　to　a　UV-Vis　detector.　The　results　show　that　by　adjusting　the　infuse　flow　rate,　the　mixing　process　can　be　accurately　controlled.　©　2009　Science　in　China　Press　and　Springer-Verlag　GmbH.