Structural　parameters　are　the　fundament　of　structure　damage　identification　and　the　precondition　of　structure　performance　evaluation.　Static　load　test　of　structure　is　straightforward　and　reliable,　and　it　is　an　importance　method　and　approach　to　gain　correct　structural　parameters　of　the　actual　structure.　In　this　paper,　the　identification　of　structural　segment　flexural　rigidity　parameters　is　studied,and　it　is　realized　by　an　optimization　method　based　on　the　static　load　test　displacement,　angular　distortion　and　curvature　responses.　Establishment　of　the　appropriate　optimization　objective　function,　and　its　algorithm　and　realization　procedure　are　described　in　details.　The　influence　of　various　static　load　testing　responses　to　the　structural　parameters　identification　result　is　studied　by　numerical　example　analysis.　The　method　is　thereafter　successfully　applied　to　a　real　prestressed　reinforced　concrete　beam　that　was　statically　load　tested　and　the　actual　structural　parameters　are　gained,　and　those　demonstrate　the　method　proposed　in　this　paper　is　correct　and　feasible.