A　method　for　the　determination　of　matrine　and　rotenone　residues　in　aquatic　products　by　liquid　chromatography-electrospray　tandem　mass　spectrometric　method　was　described.　The　analytes　were　extracted　from　homogenised　tissue　using　acetonitrile-ammonium　acetate　buffer.　After　adding　sodium　chloride　to　separate　to　the　analytes　by　salting-out,　the　n-hexane　was　used　to　defat.　Rapid　chromatographic　separation　was　achieved　on　Agilent　Zobax　SB-C(18)　column　in　the　mobile　phase　composition　of　20　mmol/L　ammonium　acetate　buffer　consisting　formic　acid　as　ion-pair　agent　and　acetonitrile.　Detection　is　carried　out　using　poistive-ion　electrospray　tandem　mass　spectrometry.　The　method　is　accurate,　specific　and　sensitive　for　the　determination　of　matrine　and　rotenone　residues　in　aquatic　products　in　the　concentration　range　of　0.　001　-　0.　05　mg/kg.　The　average　recoveries　and　the　relative　standard　deviation　ranged　from　82.　3%-96.　1%　and　6.　1%　-　12.　6%,　respectively,　in　spiked　tissue　samples　at　concentration　levels　ranging　from　0.　002　mg/kg　to　0.　2　mg/kg　of　each　analytes.　The　limits　of　detection　(LOD)　of　matrine　and　rotenone　were　0.　86　and　0.　53　mu　g/kg　respectively.　This　method　is　suitable　for　the　determination　of　matrine　and　rotenone　residues　in　aquactic　products　and　the　pharmacokinetics　study.